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We mutated the E2-box sequence from CACCTG to CGAATG so that δEF1 could not bind to the mutated site.
The ability of the AVFluIgG01 and AVFluIgG03 to bind to the mutated rHA proteins was detected by IFA.
In the absence of the C-terminal tail, Dsh did not bind to the mutated CKIα (data not shown), which strengthens our hypothesis that the C-terminus of CKIε does contact Dsh, but is not required in for interactions with wild-type CKIε.
B-R3 does not bind to the mutated receptor.
Molecules that can reactivate cell death in p53-mutant cells in a p53-dependent manner have been selected based on their ability to either kill the cells (phenotypic screening) or bind to the mutated p53 protein and restore a functional p53 conformation (biochemical screening) [ 11, 12].
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However, the active metabolite that binds to the mutated ligand-binding domain of the human estrogen receptor is 4-hydroxy-tamoxifen (4-OHT).
For example, mutations in the intracellular domain linked to internalization deficiencies are overrepresented in gefitinib sensitive cell lines, even though gefitinib binds to the mutated EGFR with the same affinity [12].
This may facilitate desaturation of shorter acyl chains bound to the mutated ACP if other aspects of the desaturation reaction do not depend on acyl chain length.
These data are in complete agreement with previous reports that Tus binds to the same mutated Ter with 4 6 fold lower efficiency [12. 13] as well as our own calculations of Tus: Ter and Tus: mutant Ter binding efficiencies and off rates (Table S1).
The chimeras bind to the cell's DNA alongside the mutated base and fool repair enzymes into fixing it.
Variants and/or mutated proteins bind to the transferrin receptor and reduce its affinity for iron-loaded transferrin.
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