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ECs can also bind to the matrix through the binding of Ang-1 to integrins, which can mediate some of the effects of Ang-1 without Tie2 phosphorylation [ 146, 151].
A proapoptotic protein produced in response to deoxyribonucleic acid fragmentation, p53, can bind to the matrix metalloproteinase 2 gene promoter and cause increased gene expression.
The His-tagged protein was allowed to bind to the matrix for 1.5 h at 4°C and eluted with an imidazole-gradient (25 300 mM) in buffer A, B and C, respectively.
Casein was cleaved into acidic peptides that were very likely to bind to the matrix by both phosphate moieties and acidic amino acids.
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Bennett, J. S., Chan, C., Vilaire, G., Mousa, S. A. & DeGrado, W. F. Agonist-activated αvβ3 on platelets and lymphocytes binds to the matrix protein osteopontin.
The mathematical model presented takes into account the viscoelastic properties of the polymer and the state of the dispersed drug: free or chemically bound to the matrix.
TGF-β1 was both conjugated through the PEG linker and bound to the matrix via physical affinity, delaying the release rate of TGF-β1 up to 10 days.
Tagged proteins bound to the matrix were released by boiling in electrophoresis sample buffer.
Under these conditions, the Oto protein elutes in a distinct peak from Oto-modified Wnt3a (not shown), indicating that Oto and Wnt3a bound to the matrix independently.
At steady state, each molecule of VEGF that binds to the matrix is matched by a molecule that unbinds.
RNA is subsequently bound to the matrix of the column, washed to remove contaminants and then eluted with molecular biology grade water.
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