These results strongly validate that a small molecule that binds to the protein protein interaction pocket distal from the active site disrupts the interaction of CDC25B phosphatase with CDK2/Cyclin A substrate and inhibits its phosphatase activity.
Thus we hypothesized that JMJD2A might bind to the ARHI promoter by interaction with E2Fs (mainly E2F1 and E2F4) and HDACs (mainly HDAC1 and HDAC3).
In addition, enzymes may bind to the membrane through specific interaction with lipids in the membrane, such as PI 3-P, PI 4-P, PI 3,5-P2 and PI 4,5-P2 through PH, PX, FYVE or ENTH domains (Strahl and Thorner 2007).
Cdc42 binds to the Cdc42/Rac interaction and binding sequence (CRIB) (residues 74 87) of Pak2, which overlaps with the autoinhibitory domain (AID).
This interaction was highly specific since ScKap95 did not bind to the Nup116 348 458 10xFA mutant and no interaction between FG-coated beads and 3xGFP could be detected.
The appendages do not depend on allosteric changes within the core to promote binding because AP-2 can be quantitatively affinity purified with immobilized appendage binding motifs or immunoprecipitated with mAb AP.6 (Chin et al., 1989), which binds to the platform interaction site of the α appendage.
Neither Ub4 nor mono-Ub, which, respectively, binds to the ubiquitin interaction motifs of Rpn10 [ 34] and N-terminal segment of Rpn13 [ 35], significantly changes the rate of MBP hydrolysis by the proteasome.
The result showed that the phage bound to ES cells by protein-protein interaction, which is consistent with the earlier report that peptides isolated from phage display often bind to the sites by protein-protein interaction [34], [35].
The next incubation followed addition of streptavidin-coated microparticles, so the sandwich complex could bind to the particulate solid phase via interaction of biotin and streptavidin.
Mechanistic studies also indicate that knockdown of HDAC1 increased histone acetylation levels at the Klf4 promoter region and that both HDAC1 and Klf4 can bind to the Klf4 promoter without direct interaction at the protein level (Supplementary Figure S4E).
