Sentence examples for bind to the column from inspiring English sources

The supernatant was allowed to bind to the column overnight at 4 °C.

Briefly, rabbit antisera were diluted 1∶2 and passed through protein G columns enabling antibodies to bind to the column.

Protein that did not bind to the column was kept for analysis of the inner core of the LB.

Thus, this protein cannot bind to the column, probably due to degradation of the affinity tag.

C1q does not bind to the column, but contaminants were removed.

At pH 8.0, the Nf xylF did not bind to the column.

When we characterized the binding of the complement regulators C1INH, C4BP, and factor H by Western blotting (Fig. 1B), we found that in the presence of C1q, C1INH, factor H and C4BP bound to the column, but after C1q depletion, binding of C1INH was no longer seen, suggesting that the protein bound to CS-A in association with C1q.

M, protein marker; hLF, human lactoferrin standard; TG, milk samples from transgenic cows; P1 and P2, rhLF eluted from the column; FT, protein fraction that was not bound to the column.

SCARB2 bound to the column was eluted in a buffer containing 50 mmol/L imidazole.

Proteins bound to the column were then eluted with elution buffer (column buffer with 1 M NaCl).

MtbHadAB complex bound to the column was eluted with a 0.05 1 mol/L linear gradient of NaCl.