We found that CPX binds to the blocked SNAREΔ60 with a binding affinity of 15.2 ± 1.4 µM, matching well the Kd ∼16 µM reported in Kümmel et al. (2011).
The Shanghai team used a crystallized form of the CCR5 receptor, bound to the blocking drug Maraviroc, to determine the receptor's structure.
The antibody that is bound to the blocking protein is no longer available to bind to the epitope present in the protein on the tissue.
We have now additionally carried out the ITC experiments with the minimal functional domain (CPX26 83) and find that this truncated version also binds to the blocked SNAREΔ60, albeit with slightly weaker affinity (Kd = 23.9 ± 0.1 µM) compared with full-length CPX.
Based on our previous studies, SELPs tended to assemble into micellar-like particles with silk blocks buried in the core, thus, the hydrophobic compounds likely bind to the silk blocks.
DNA-binding proteins bind to the duplex and block strand exchange, thereby suppressing the fluorescence elevation.
Cetuximab, a human-mouse chimeric IgG1, and panitumumab, a fully human IgG2, are monoclonal antibodies (moABs) that compete with EGFR's ligands and specifically bind to the receptor, blocking ligand-induced downstream signaling [ 2].
The C84 fullerene has an approximate diameter of 8 Å. Ideally, an ion channel blocker design would have flexible side chains which can bind to the channel and block the entrance to the pore.
Agonist molecules can bind to the pore and block ionic conduction.
HSV infection stimulates the immune system to produce predominantly glycoprotein D-specific antibodies: these neutralizing antibodies bind to the virus and block its ability to infect cells.
