Sentence examples for bind to the affinity from inspiring English sources

It was in accordance with the fact that SiCYS-C could not efficiently bind to the affinity column coupled with papain.

The serum IgG was allowed to bind to the affinity tagged column at 4°C for 45 min. The column was then washed with buffer containing 100 mM Tris-HCl (pH 7.9).

The control dataset was generated by performing several pull downs in cells that expressed the vector alone to identify proteins that non-specifically bind to the affinity beads used in the purification.

In the open-well configuration, secreted proteins are free to diffuse into the surrounding media or adjacent wells as well as bind to the affinity ligands supported on the substrate.

This means that at low ligand concentrations, only the high-affinity-binding kinases are not trapped on the affinity material anymore as they are bound to the ligand, whereas at higher ligand concentrations, also the lower-affinity kinases are unable to bind to the affinity material anymore.

In assay (ii), subsequent screening of 88 different buffer ligands/additives with 3xflag-LRRK2 purified with lysis, wash and rinse buffers in which Tris pH 7.5 was replaced by Hepes at pH 6.8 and still bound to the affinity beads.

When the activated ER bound to the affinity region, the molecular lost its swingable function, the electron transfer from the photo-excited dye to the SCE was consequently suppressed.

ProteoPlex assays of (i) 88 buffers and (ii) 88 buffer ligands/additives were performed in 96-well plates as given in ref. 29 to determine the optimal conditions for the stability of 3xflag-LRRK2 bound to the affinity beads (i.e., the optimum lysis/binding buffer).

Proteins bound to the affinity matrix were packed to empty 10 ml columns (MoBiTec) and washed three times with MBP-buffer.

The proteins bound to the affinity columns were then eluted by addition of 50 mM and 1 M NaCl, and then 10 mM glutathione.

Higher concentrations of competitor result in lower analyte retention times since the competitor also binds to the affinity column.