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Such analysis indicates that these reagents bind to subsets of mannosyl antigens on the envelope (env) protein.
We induced the TFs that bind to subsets of a given gene list using the "Functional Annotation Tool" in DAVID [ 12, 13].
We tried to extend the core transcriptional regulatory network based on the combination of the core-bound genes and the TFs that bind to subsets of the core-bound genes.
The identification of mRNA targets of individual Puf proteins has revealed that Puf proteins typically bind to subsets of mRNAs that are functionally or cytotopically related and located within macromolecular complexes.
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As one possible model, it is plausible that SMC1 phosphorylated at Ser966 specifically binds to subsets of cellular proteins, generating proapoptotic complex.
CL-K1 also bound to subsets of fucosylated glycans, including those containing Lewisa, Lewisy and blood group B antigens as well as to a selection of structures containing blood group H antigens.
BIM, BID and PUMA are 'promiscuous', binding avidly to BCL-2 and all its pro-survival homologues, whereas other BH3-only proteins, such as NOXA and BIK, bind only to subsets of pro-survival proteins.
Recent studies in yeast and in mammalian cells have emphasized that hundreds of proteins bind to specific subsets of mRNAs.
Depending on cellular contexts, such as hormone stimulus or the cell's differentiation state or cell type, a TF can bind to different subsets of the TF's potential binding sites and regulate different gene expression programs [ 1].
Bad and Noxa, on the other hand, bind to complementary subsets of anti-apoptotic factors, with Bad interacting strongly with Bcl-2, Bcl-xl and Bcl-w and Noxa binding to Mcl-1 and A1.
We induced the TFs that bind to the subsets of the core-bound genes with DAVID tool [ 12, 13] and analyzed the transcriptional network based on the induced TFs.
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