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Soluble biotinylated CNE did not bind to immobilized fibrinogen.
As is usually done, we had selected anti-PLAP scFv by allowing the phage library to bind to immobilized PLAP and eluted with high pH.
In this work, we present data supporting that PRG4, in its native and recombinant forms, can bind to immobilized TLR-2 and TLR-4.
In the aforementioned study, BSA at concentrations as high as 2.5 mg/ml was not shown to bind to immobilized recombinant MIF [ 17].
Control enzyme-linked immunosorbent assays (ELISA) showed that at least a subset of the antibodies in the polyclonal commercial preparation could bind to immobilized 18-mer 1 (indirect ELISA).
SPR methods were used to test if purified EsxA, or EsaB, a small soluble protein that appears to regulate the production of the T7SS substrate protein EsaC [ 11], bind to immobilized EssB-N (Supplementary Figure S1).
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Furthermore, investigations of the binding reaction using SPR showed that fibrinogen bound to immobilized Col I with a high on-rate.
As shown in Figure 1(B), all of the truncated proteins bound to immobilized AtCaM7, suggesting that the CaM-binding site was located within the kinase domain of BRI1.
The binding is specific to the extent that fibrinogen at nanomolar concentrations bound to immobilized native collagen, but not denatured collagen.
(d) Affinity purification of membrane proteins bound to immobilized salusin-β.
Furthermore, equine monocytes bound to immobilized ePsel-IgG in a time course and dose dependent manner.
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