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Figure 2 A shows the average number of TFs that bind to genes of different ages, with a clear increase in TF binding for old relative to young genes.
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We find that the ABF1 transcription factor binds to genes encoding subunits of all three complexes.
Based on the same principle, artificial transcription factors (ATFs) have been generated by fusing various functional domains to a DNA binding domain engineered to bind to the genes of interest, thereby modulating their expression.
TFs form a protein complex that binds to gene promoters during the initiation of transcription.
In our study, we found that HBc can bind to 64 gene promoters of the MAPK pathways, and 41 gene promoters of the Wnt/β-catenin signaling pathways.
Importantly, both activating E2Fs and Myc are recognized as essential cell cycle regulators and bind to promoters of genes important for driving cellular proliferation [ 33].
Regulators such as Stb3 and Sfp1 bind to one set of genes but affect expression of the other set of genes.
For example, miR-4282 was predicted to bind L1 genes of 15 HPV subtypes.
PHA-4 has a broad role in regulation of gene expression but can preferentially bind to specific set of genes under distinct developmental or environmental conditions.
We find that all complexes bind to promoters of housekeeping genes in ESCs and NPCs with NSL complex members occupying the majority of the target genes, while MOF and MSL proteins bind NSL-bound genes in a more restricted manner.
Expression of immunoglobulin (Ig) genes requires octamer binding proteins (Oct) to bind to the promoters of Ig genes.
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