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The library was selected for scFvs that bind to extracellular loop epitopes on either of two known immunogenic S. mansoni membrane proteins, Sm23 and SmTsp2, both of the tetraspanin protein family.
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Secreted Maspin could bind to extracellular matrix components.
For instance, proton and peptide toxins bind to extracellular pore domain of the channel, while capsaicin binds to its transmembrane domains.
The α and β extracellular domains cooperatively bind to extracellular matrix components such as collagen, laminin, and fibronectin.
These integrins can also bind to extracellular matrix components such as serum, fibrinogen and fibronectin.
Gonadotropins bind to extracellular receptors, the LH-R and FSH-R.
However, the cytotoxicity was unchanged by adding EGTA, which can only bind to extracellular calcium.
DAMPs are released from necrotic cells and bind to extracellular receptors on surrounding cells.
Since the extracellular side of the channel appeared to be facing the pipette, we used mAb56, a monoclonal antibody reported to bind to the extracellular loops of KV10.1 and inhibit current [12].
Activation of chemokine receptors occurs in a two-step fashion, in which the globular part of the chemokine ligand binds to the extracellular loops of the receptor and the flexible chemokine N-terminus subsequently enters a defined pocket between the transmembrane helices (Monteclaro & Charo, 1996).
This "tethered ligand" is able to bind to the second extracellular loop of the receptor, leading to the transduction of signal events.
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