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These fragments can bind to complementary transcripts and downregulate their expression via an RNA interference-like mechanism, as explained above.
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Activated RISC then binds to complementary transcript by base pairing interactions between the siRNA antisense strand and the mRNA.
miRNAs are post-transcriptional regulators that bind to complementary sequences on target messenger RNA transcripts, usually resulting in translational repression and gene silencing.
MicroRNAs (miRNAs) are post-transcriptional regulators that bind to complementary sequences on target messenger RNA transcripts (mRNAs), usually resulting in translational repression or target degradation and gene silencing [ 8- 10].
miRNAs are post-transcriptional regulators that bind to complementary sequences in the 3′ UTRs of mRNAs.
miRNAs bind to complementary sites on target mRNA sequences and induce cleavage of the transcript or repress translation (Ambros, 2004; Bartel, 2004).
miRNAs are small (~22 nucleotides) non-coding RNAs that bind to complementary sequences in the untranslated regions of target mRNAs and contribute to gene regulation by reducing mRNA translation or destabilizing transcripts [ 32– 32].
Usually 25 bases in length, they bind to complementary sequences of RNA by standard nucleic acid base-pairing.
They typically bind to complementary loci in the 3′ untranslated region (UTR) of mRNA and prevent translation to mature protein.
For amplification of a band in RAPD analysis same primer has to bind to complementary sites on both strands.
For animals, the seed sequences of miRNA can bind to complementary sites on 3'UTR of its targeted gene.
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