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Gp150 amino acid residues 1-151 fused to IgG1-Fc failed to bind to cell surfaces (Figure 5B), as did fusions of residues 1-250 and 1-450 (data not shown).
The ability of lectins such as ConA to bind to cell surfaces depends on the degree and forms of glycosylation for different cells.
Secretory Asm has been previously show to bind to cell surfaces (Dhami & Schuchman, 2004; Schissel et al, 1998b), allowing the enzyme to be active on the cell surface.
Antibody fragments that bind to cell surfaces have largely been selected from phage-displayed libraries, with examples including those that bind to ovarian, breast, and hepatocellular carcinoma cells [ 24- 26].
Quantification of fl-OPN is also complicated by the potential for the protein to bind to cell surfaces via integrins, or to bind to circulating factors such as complement Factor H [ 37].
This was explained by the fact that Lgi1 is secreted from neurons and can then bind to cell surfaces, as shown in neuroblastoma and COS7 cells (Sirerol-Piquer et al., 2006) and now demonstrated in HEK293 cells.
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Because ADCs are designed to bind to cell surface receptors, trough circulating drug concentrations are typically low.
These molecules bind to cell surface receptors and are subsequently internalized by endocytosis, resulting in cell death.
Understanding how pathogens bind to cell surface proteins can lead to the design of novel carbohydrate-based drugs and diagnostic and therapeutic agents.
Aptamers are a class of therapeutic oligonucleotides that bind to cell surface receptors with high affinity and specificity (Levy-Nissenbaum et al. 2008).
Epithelial cells cultured on stiff matrices exhibit increased contractility thus enabling release of TGFβ from its latent complex thereby increasing the amount of active TGFβ accessible to bind to cell surface receptors.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com