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The water serves to carry the dyes into the fibres, where acidic dyes bind to basic sites and basic dyes bind to acidic sites.
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These acidic GAG epitopes bind to basic residues in LG domains via electrostatic interactions.
SRB is a bright-pink aminoxanthene dye with two sulphonic groups that bind to basic amino-acid residues under mild acidic conditions, and dissociate under basic conditions.
Naphthalene-sulfonic acid derivatives were found to bind to arginine only, as opposed to expected binding to all basic sites (Arg, Lys and His).
Nef Glu160 of the motif binds to basic residues on both σ2 and α.
KLF13 is also a transcription repressor that binds to basic-transcription element [ 65].
At this time the freshly precipitated fibres, prior to the drying and collapse of their gel structure, still contain some water and solvent and are therefore open to the entry of basic dyes that bind to acidic sites on the polymers.
Noncovalently bound complexes between basic sites of peptides/proteins and sulfonates are studied using Matrix Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry.
Noncompetitive inhibitors often bind to allosteric sites in enzymes.
Carrageenan and glycan bind to different sites on AFAL.
In particular, Gln104, Arg105, and Asp108 bind to a basic patch on α.
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