The libraries were designed to cover the packing of the ligand in the libraries around a heme group after being bound to a template attached to a cellulose backbone.
Finally, (d) a barrier to exonuclease RecJF created by SA bound to a template biotin residue was shifted forward approximately two nucleotides as a result of RT pushing against the barrier from the upstream side.
Although implicit in this study, this complex is also significant in another respect as it also provides a structural glimpse of a member of the AEP family, that includes eukaryotic replicative DNA primases, bound to a template-primer substrate, thus providing insights into how these essential enzymes bind to and extend a DNA primer strand.
Each primer was designed to bind to a complementary template immediately adjacent to the mutation site.
The antisense DNA can bind to a cell's RNA template for the protein, thus strangling its production.
DNA polymerase requires a primer that can bind to a known region of the template strand.
NS5B was found to bind to a nonstructured and a structured RNA template in different modes.
Our findings suggest that the CTD does not catalyze a specific template switch or bind to a specific nucleic acid structure as its primary mechanism.
Here, DNA polymerase molecules, bound to a DNA template, are attached to the bottom of 50 nm-wide wells termed zero-mode waveguides (ZMWs).
Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is.
