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Using the framework described above we can define ϕ for the simplest model of interest in this study, in which the morphogen activator GliA (A), and repressor GliR (R), bind to a single DNA binding site (GBS).
These data suggest that Ca2+ must bind to a single specific metal binding site before phosphodiesterase can be fully activated.
Brunelli and colleagues [ 48, 49] have shown that 17β-estradiol could bind to a single specific and saturable binding site of ApoB100 and prevent the misfolding of its structure on electronegative LDL without changing the electronegativity and phospholipase activity of LDL.
The pairing of the chemokines to their receptors has been carried out, mainly by receptor binding assays, and has identified receptors that are specific (bind to a single ligand) or shared (bind more than one ligand).
Repression of mRNAs may be also increased by multiplicity (several binding sites in a transcript for a single miRNA) and cooperativity (several miRNAs bind to a single transcript) [ 25].
To address that issue, the GoodSIRS team engineered chemical agents called antibody maytansinoid conjugates (AMCs) to modify antibodies to bind to a single cytokine.
Similar(17)
The differential affinity of the enzyme for each site, its capacity to bind to a single-stranded structure at the attC site and the recombination observed with single-stranded substrates unambiguously confirm that it constitutes an important intermediary in the reaction.
In membranes isolated from glands of non-irradiated animals, H-NMS bound to a single binding site (see methods), with a maximal binding capacity (Bmax) of 39±12 fmol mg−1 protein and a dissociation constant (p Kd) of 9.9±0.11 n M.
The finding that the dimeric C-terminal actin-binding site of talin binds to a single actin filament explains why this domain fails to bundle actin filaments under the conditions used in this study.
Scatchard plots of the binding data indicate that [18F]-9 binds to a single high affinity site on AD homogenates and Aβ1 42 fibrils (Fig. 5C,D).
The quenching mechanism was suggested to be static quenching according to the fluorescence measurement and the linearity of Scatchard plot indicated that daunorubicin bound to a single class of binding sites on HSA.
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