Overall, the number of binding sites was less than unity (about 0.7 per mol of sites), which may reflect some endogenous substrate bound to a fraction of the purified protein (see below) and/or an overestimation of the protein concentration.
To determine whether TAF15 binds to a fraction of the U1-Sm snRNP or whether it is a component of a new, not yet identified minor U1 snRNP, co-immunoprecipitation experiments were performed (Fig 1D).
Successful targeting of PPIs is also made possible due to the ability of the small molecules to bind to a small fraction of the residues contributing to the interface, also known as binding hotspots, which contribute the most to the free energy of interaction.
Perhaps ZLD binds to a large fraction of its sites because there simply is no condensed chromatin in the early embryo.
Why MLL1 protein binds to a major fraction of gene promoters but with limited functions is still unknown at this moment.
Hypersensitive DNase, H3K4me2, H3K4me3, H3K9ac and H3K27ac, which are related to transcriptional activation, bind to > =80% H2H pairs; while H3K27me3 and H3K9me3, which are associated with gene silencing [ 26 ], are only bound to a small fraction of H2H gene pairs.
We performed a genome wide analysis to identify c-Myb target genes and although we demonstrated that c-Myb is bound to a substantial fraction of the genes, our numbers are consistent with other similar studies.
Thus, at very low Hg concentrations in serum, Hg may bind to a variety of different protein fractions, whereas the stronger competition and reactivity of the selenol group in selenoproteins explains why more Hg was observed in the SeP-containing fraction at higher Hg exposure concentrations.
In vitro binding studies (Hopkinson et al, 1999) have shown that Foscan initially binds to a heavy, nonlipoprotein fraction (not albumin) in human plasma, which does not fluoresce, and subsequently redistributes to the plasma lipoproteins.
For over a millennium, Chinese women of all ages and social classes have bound their feet to a fraction of their original size, suffering great pain and hardship during the process.
