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The fact that some BORIS isoproteins can bind the CST promoter and activate transcription while others can bind the same promoter but do not display transcriptional activity (Fig. 8) suggests that BORIS isoforms, similarly to CREM, can perform opposing functions with the same DNA target.
We do not know if CepR and CciR bind the same promoter motif.
It is possible that one or more was identified because of its similar binding motif without actually playing a role in SIRT3 expression; it is also possible that they are important in different contexts, or, when they are co-expressed, multiple factors may bind the same promoter element to activate gene expression with different strengths (Takahashi et al., 2008).
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Basonuclin's high affinity binding site overlaps with the binding site of a dedicated and ubiquitous Pol I transcription regulator, UBF, suggesting that their binding might interfere with each other if they bind to the same promoter.
Interestingly, in H. jecorina the cellulase activator, Ace2, has been shown to bind to the same promoter motif as XlnR, while the Hap2/3 complex opens the chromatin structure, promoting nucleosome reassembly and derepression [ 15].
To confirm that BES1 and RD26 can bind to the same promoter regions at the same time, we also performed ChIP reChIP with chromatin prepared from RD26OX, rd26 anac019 anac055 anac102 (rdQ) or BES1 RNAi plants in which the BES1 level is reduced27 (Supplementary Fig. 9).
They often bind to the same promoter under activating conditions, and yet their individual contributions to expression of the gene vary widely.
In other words, if two different TFs bind on the same promoter regions they would very likely act together to direct the expression of their target genes.
4) You claim that MP recruits BRM/SYD to chromatin based on the finding that they bind to the same promoter regions using ChIP-PCR.
However, there are exceptions where two different σ factors bind to the same promoter (Weber et al., 2005; Wade et al., 2006; Typas et al., 2007).
Thus, an overlapping DNA binding site may not be a prerequisite for SerRS to antagonize c-Myc, as long as both SerRS and c-Myc bind to the same promoter.
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