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We conclude that REF and UIF simultaneously bind the same mRNA molecule in vivo.
The hairpins show good matches to human and rodent mature sequences, which could potentially bind the same mRNA as their respective human or rodent counterparts.
Because miRNAs can regulate mRNA expression in both a negative manner and a positive manner (Vasudevan et al. 2007) and because many miRNAs can bind the same mRNA (Saetrom et al. 2007), it is difficult to draw conclusions from miRNA studies without infomation on the concurrent mRNA(s) expression pattern.
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Moreover, multiple miRNAs can bind to the same mRNA and coordinate its expression [ 2].
Indeed, inside the cell, mRNAs (microRNA targets) exist as ribonucleoprotein particles or mRNPs, and second, all proteins normally associated with mRNAs transcribed in vivo are absent or at least much different from that bound to the same mRNA in an in vitro system or following the microRNAs transfection into cultured cells.
Other basic factors highly related to active targets are miRNA cooperation (17), where a plausible effect in regulation is identified when several miRNAs are simultaneously bound to the same mRNA (rather than separately), and thermodynamic stability, where favourable energy is determined among the bound and unbound RNA double strand (18).
Depending on the complementarity of the miRNA to its target, one miRNA may bind several regions on the same mRNA or on different mRNAs (Filipowicz et al, 2008).
DND1 has been shown to bind to the 3'-UTR of mRNAs to displace miRNAs that bind to adjacent sites on the same mRNA [8].
Even the same mRNA sequence can be regulated by several micro-RNAs bound up to it [5].
The same mRNA was detected with both QD525 (a) and QD585 (b) probes.
The mouse TrkB mRNA is one of only a few eukaryotic mRNAs to contain two IRESes within the same mRNA.
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CEO of Professional Science Editing for Scientists @ prosciediting.com