Sentence examples for bind the putative site from inspiring English sources

To investigate whether NF-κB could bind the putative site on the HIF-1α promoter, we performed EMSAs using nuclear extracts derived from HEK-293 cells which had been transfected with the individual NF-κB subunits.

Along with these loops, the N-terminal helix in the OTUD1 and OTUD3 catalytic domain and the structurally equivalent C-terminal helix of the OTUD2 catalytic domain form the putative sitesithathat binds the proximal Ub.

The putative site upstream of MXAN_0524 (also high on the list in Additional file 3) produced a single complex that was inferred to have 2 His10-MrpC2 bound based on its migration, and 2 sites were subsequently verified (see below).

The putative sites upstream of bsgA and pkn8 produced primarily a complex inferred to be bound by 1 His10-MrpC2, although a small amount of a complex inferred to have 2 His10-MrpC2 bound was observed for bsgA.

Both CREB1 and ATF4 proteins could bind the putative CRE binding sites to enhance their gene expressions.

We next asked whether the MEF2-TBP complex could bind the putative MEF2-TATA site.

Drawing on previous work by Jenkins et al. (2001) which showed that the binding site for haloalkane GAs in the GABAA receptor could only accommodate one molecule, they proposed that pressure reversal occurred when halothane aggregated, so fewer monomeric halothane was available to bind to the putative binding site.

Furthermore, the upstream region of MAMLD1/Mamld1 harbors a putative binding site "CCAAGGTCA" for NR5A1 (alias, SF-1 and AD4BP) [4] that regulates the transcription of a vast array of genes involved in sex development [5], and NR5A1 protein has been shown to bind to the putative target site and exert a transactivation function for Mamld1 [4].

The heterodimer will then bind to the putative DRE site(s) on the promoter region of the ACHE gene, suppressing the expression of AChET transcripts.

To demonstrate that nuclear proteins present in MDA-MB-231 cells bind to the putative GAS site in the distal LKB1 promoter, EMSAs were carried out.

To investigate whether AmphiFoxD protein can bind to putative sites within the conserved region of AmphiVent1 and AmphiVent2 promoters, double-stranded oligonucleotides derived from the corresponding regions of each promoter were tested by in vitro DNA-binding assay (electrophoretic mobility shift assay, EMSA).