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MCF-7 cells possess low endogenous cell-surface (pro -uPA levels and bind small amounts of cell-surface pro -uPA].
This effect is likely due to the incapacity of Lhcb proteins to fold in the absence of xanthophylls [ 37], while Lhca proteins can also bind small amounts of β-carotene [ 40, 41].
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Nuclear imaging techniques such as SPECT provide the possibility of functional analysis of in vivo processes, allowing the detection of even small amounts of bound ligands down to picomolar concentration [25].
However, only small amounts of CAND1 bind to Cul1 in cells, despite low basal levels of Cul1 neddylation and approximately equal cytoplasmic endogenous protein concentrations of CAND1 and Cul1.
An alternative explanation for the background signal of asMYO5A ARE measured in control cells may be the existence of small amounts of AR constitutively bound to the chromatin, as described for another transcription factor, cyclic AMP-responsive element-binding protein (CREB) [ 41].
The data suggest that SaCBM42 binds to small amount of arabinosyl side chains that were left on debranched arabinan and thus increases the enzyme concentration around the substrate.
In answer to the reviewers' question, any CTB that is internalized when cells are cultured with 2F-Fuc could reflect CTB molecules that are not bound to fucosylated proteins, or possibly CTB bound to a small amount of fucosylated proteins that might remain present even with 2F-Fuc inhibition.
All cells expressing mutant kidney band 3, detected with the αrbB3Ct antibody, also bound a very small amount of FITC-BRIC6, suggesting that a small proportion of S667F-kB3 is able escape the ER and to reach the cell surface.
Currently, judges are bound by a sentencing structure that requires minimum sentences of one year for possessing small amounts of cocaine or heroin, for example.
Small amounts of hornblende are often present.
Broccoli contains small amounts of carcinogens.
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