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These metalloregulatory proteins, in principle, sense the intracellular levels of specific metal ions by binding them to a metal binding site, which leads to conformational changes affecting the regulator's ability to bind operator sites in regulatory DNA regions [ 7].
In POS, this is realized by BIND operator.
The operators coming after PARTITIONs will be cut off and moved to the BIND operator.
The FOREACH1 operator following PARTITION1 and the FOREACH2 operator following PARTITION2 are both cutoff and reconnected to BIND operator.
In the process of building topology plan, when a PARTITION operator is visited, it will first look backwards to see whether there is a BIND operator following it.
BIND must be used immediately after two or more PARTITION operators, that is, BIND operator must have PARTITION operators as its parents in a logical and physical plan.
Similar(51)
PARTITION and BIND operators realize stream grouping mechanisms.
The majority of transcription activators characterized to date are dimeric proteins that bind operators upstream of the promoter −35 element and directly contact the RNAP α subunit (Ebright, 1993), the σ4 domain positioned at the −35 element, or both (Nickels et al., 2002; Dove et al., 2003; Jain et al., 2004).
(ii) Kd, constant of dissociation, inverse concentration at which 50% of the ligand is bound, which describes how TF-A binds its ligand A, (iii) Kb, binding constant, inverse concentration of the TF at which 50% the available binding sites are bound, which describes how TF-A binds operators of the matching type(s).
Upon in vitro γ-irradiation the repressor losses its ability to bind the operator DNA sequence due to damage of its DNA-binding domains.
They bind DNA operator sequences responding to the effector molecule.
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