Importantly, in solution the effector fragment again was bound equally to the mutant and to the parent K-ras.
The observation that the three UIM sites in ataxin-3 bind equally to a single site of the parkin Ubl domain suggests a multi- or polyvalent ligand binding mode.
D) The reviewer raised an important question concerning the ability of Nap1 to bind equally to all H3 types used in the assay.
As Bag5 is made up of five BAG domains, it was necessary to determine whether all the five domains bind equally to GRP78/BiP.
However, IgM antibodies were found to bind equally to unclustered and clustered AChR in a few samples from both SNMG and MuSK-MG.
These doubts are further strengthened by the observation that many non-RA sera with low ACPA levels bind equally to citrullinated and non-citrullinated peptides and that low ACPA levels may not discriminate between true and false positive results [ 26].
Interestingly, while the delta-PB1 mutant bound equally to all three chain types, the oligomeric wild type protein showed increased binding to linear ubiquitin and to some extent to K63-linked ubiquitin chains but not to K48-linked ubiquitin.
None of those samples that were negative on clustered adult AChR were positive for binding to clustered foetal AChR, and 36/39 of those that were positive bound equally to both forms.
Furthermore, although PIASy bound equally to wild type VHL and its K171R mutant, PIASy only induced SuVHL-L band in wild type VHL instead of the K171R mutant (Figure 2E), indicating that SuVHL-L is indeed an isoform of VHL SUMOylated on Lys-171.
All of the antibodies were positive in radioimmunoassays, some more strongly than others, and each antibody bound equally to extracts of different kinds of tissue.
