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In addition it also caused a trans to cis-amide isomerisation, known to be required for binding to the Hsp90 protein to occur, whilst protein crystallography established that such 19-BQAs did indeed bind efficiently to the N-terminal ATP-binding site of Hsp90 (Fig. 2).
Combined with our observation that H3 10mer peptides bind efficiently to the PHD1 domain (Fig. 1, Table 1), we selected the H3 10mer peptide (Ala1-Ser10) as ideal effector peptide for our experiments.
This way, the antibodies become more available to bind efficiently to the gp41 epitope, blocking viral fusion faster than the unconjugated antibody.
The 3D-QSAR and docking results revealed that compound 32 bind efficiently to the wild and mutated protein whereas, sufficient interactions were lost in compound 46.
These results demonstrated that the CC49-QDs Ab probe can recognize and bind efficiently to the unblocked TAG-72 of MGC80-3 cells.
As previously demonstrated, Nrm1p and Yox1p bind efficiently to the MBF targets, cdc22+ and cdc18+ [19], [19].
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In confirmation of the FACS study, there was minimal binding of A192; however, A192-RGD bound efficiently to the cellular surface.
Purified PLG bound efficiently to the spore surface, while plasmin showed more efficient binding in the NHS (Figure 2A), suggesting less existence of PLG in the NHS.
Both P2T1 and P2T3 bound efficiently to the D loop substrates but showed reduced single-stranded DNA binding efficiency compared to the full length PALB2 protein.
That the probe binds efficiently to the minor groove near the deformed site was evident from spectroscopic studies.
ZNF198, however, bound efficiently to the intact LCH ternary complex (Figure 3B, lane 2; Figure 3C, lane 1).
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