Sentence examples for bind at the substrate from inspiring English sources

The results showed that the xenobiotics bind at the substrate binding site.

Modeling showed that the catechol moieties in AG538 could be superimposed over Tyr1162 and another tyrosine in the activation loop (Tyr1158), suggesting that AG538 could bind at the substrate phosphorylation site.

Inhibitor core scaffolds were superimposed on Tyr1162, and from this a series of hydroxynaphthalene and hydroxyindole methyl esters and amides predicted to bind at the substrate phosphorylation site were designed.

The hypothesis that tyrphostins would bind at the substrate phosphorylation site was supported by Dixon plots demonstrating competition with a peptide substrate for several compounds including AG18 and AG99; however, no evidence was provided to demonstrate that the compounds were noncompetitive with respect to ATP.

Stebbins and colleagues have also reported using a competitive binding assay for the identification of inhibitors targeting a scaffolding site in JNK, but although these inhibitors were shown to compete with a substrate protein, they do not bind at the substrate phosphorylation site.

These assays rely on the net displacement of a probe to measure ligand binding, and therefore using a probe that binds at the substrate phosphorylation site should enable the exclusive identification of substrate phosphorylation site inhibitors.

In this conformation, it is possible to model a molecule of GTP bound at the substrate site, with most of the substrate-binding and catalytic residues in the same positions as in AC (see Figure 4 of [ 9 ]).

Although this analysis supports earlier biochemical data and the model suggesting AG538 binds at the substrate phosphorylation site, other tyrphostins with similar structures were shown to be competitive with both peptide substrate and ATP.

The structure of erbstatin also resembled the styryl pharmacophore found in peptide inhibitors incorporating dehydrophenylalanine in place of tyrosine, further supporting the hypothesis that it bound at the substrate phosphorylation site.

The resulting nanomolar-potent inhibitors that were developed bind at both the substrate binding pocket and the allosteric site, and can be used as chemical probes to further elucidate the function of this protein.

It should also be noted that although some inhibitors targeting substrate docking sites show competition with peptide or protein substrates, they are considered outside the scope of this review because they do not bind at the protein substrate phosphorylation site.