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The CRISPR/Cas9 binary vectors were constructed as previously described (Ma et al., 2015).
Binary vectors were constructed for Agrobacterium-mediated transformation using the MultiSite Gateway LR clonase reaction (Invitrogen), as described previously (Wakasa et al., [2006]).
The pGreen binary vectors were constructed based on a previously reported strategy [ 41].
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A binary vector was constructed to express a gentian F3H cDNA (GtF3H-1[ 62], accessionono. AB193311) in CrW.
All binary plant transformation vectors were constructed by incorporating the genes of interest into pCAMBIA-1300 binary transformation vector containing plant selectable marker for hygromycin resistance [ 85].
To identify the fungus-responsive element in BjCHI1 promoter (BjC-P), a series of binary plant transformation vectors were constructed by fusing the BjC-P or its deletion-derivatives to β-glucuronidase (GUS) reporter gene.
To encapsulate sequence information for SVM training and testing, input vectors were constructed using simple binary or bi-profile Bayes Features encoding.
The LMS Cherry vector was constructed from the LMS vector by standard cloning techniques.
Binary vectors were introduced into Agrobacterium strain EHA101 [46].
Binary vectors were transformed into Agrobacterium tumefacieriens strain gc101.
A binary vector system was constructed and successfully disrupted the polyketide synthase gene pksCT in M. purpureus SM001 through the Agrobacterium tumefaciens-mediated transformation.
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