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A. rhizogenes K599 which lacks the binary vector was used as negative control for the transformation.

For expression in plants, pMDC32 Gateway binary vector was used [ 27].

The pBI121 binary vector was used as positive control (Fig. 3).

A regenerated plant from hairy root developed by A. rhizogenes harboring no binary vector was used as negative control.

Agrobacterium tumefaciens strain C58C1, transformed with the pCAMBIA1300 binary vector, was used to prepare the T-DNA-tagged lines.

Genomic DNA of regenerated plants derived from hairy roots induced by A. rhizogenes K599 harbouring no binary vector was used as negative control.

Roots of transgenic plants derived from hairy roots that were induced by A. rhizogenes K599 harbouring no binary vector were used as negative control.

Four different binary vectors were used for the tagging approach in plants.

Five binary vectors were used for switchgrass transformation and co-transformation.

For expression in plants, pMDC99 or pMDC123 Gateway binary vectors were used [ 27].

In a second approach, EHA105/pDU10.2412 (Pv010) and EHA105/pDUV10.0104 (Pv194) binary vectors were used separately to transform J1 somatic embryos.