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The patatin hpRNAi binary vector was transformed into potato plants (cv. Desiree) via Agrobacterium-mediated transformation.
Not I fragments were cloned into pART27 binary vector [ 21] and then patatin hpRNAi binary vector was transformed into potato plants (cv. Desiree) via Agrobacterium-mediated transformation.
The binary vector was transformed into Agrobacterium tumefaciens strain EHA105 (Hood 1993) by electroporation, and Srs5 mutants were transformed as reported previously (Ashikari et al. 2005).
The pART27G14 binary vector was transformed into Agrobacterium tumefaciens strain LBA4404 [ 21] using the freeze-thaw method [ 22].
Each binary vector was transformed into Agrobacterium tumefaciens EHA101 by electroporation (MicroPulser: Bio-Rad, Tokyo, Japan). A. thaliana ecotype col-1 was transformed by the floral dip method as described by Clough and Bent [ 60].
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Two different orientations of the Cab19 GUS fusion relative to the left T-DNA border of the binary vector were transformed into tomato.
Binary vectors were transformed into rack1a-1 or rack1a-2 mutants by Agrobacterium-mediated transformation [ 23].
Binary vectors were transformed into Agrobacterium tumefacieriens strain gc101.
Binary vectors were transformed into Agrobacterium tumefaciens GV3850.
The binary vectors were transformed into Agrobacterium strain GV3101.
The resultant binary vectors were transformed into Agrobacterium stain AGL1.
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