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The binary vector was designed for Agrobacterium tumefaciens to transfer the T-DNA into a plant cell nucleus (Joh and VanderGheynst, 2006).
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A set of binary vectors were designed for a genomic gene (BDV1, Figure 3A) or CDS gene expression (BDV2, Figure 3B), gene silencing (BDV3, Figure 3C), and ethanol-inducible expression (BDV4, Figure 3D), respectively.
The resulting binary vector was designated pXCGH.
The binary vector pCAMHSP was designed and mobilized into two Agrobacterium tumefaciens strains C58 and GV2260.
The binary vector was introduced into AglI by electrotransformation.
The new binary vector was named as Keller_motif_GFP vector.
The first widely used binary vector was pBIN19 [ 3].
Not I fragments were cloned into pART27 binary vector [ 21] and then patatin hpRNAi binary vector was transformed into potato plants (cv. Desiree) via Agrobacterium-mediated transformation.
N. benthamiana leaves were infiltrated with Agrobacterium tumefaciens strains containing pgR107 binary vectors that were designed to combine the advantages of A. tumefaciens-mediated transfection and viral infection.
The RNAi-HMGR construct was designed using the pHANNIBAL vector and subsequently cloned into a binary vector pART27.
Binary vectors were introduced into Agrobacterium strain EHA101 [46].
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