Sentence examples for binary vector system from inspiring English sources
A binary vector system was constructed and successfully disrupted the polyketide synthase gene pksCT in M. purpureus SM001 through the Agrobacterium tumefaciens-mediated transformation.
In this study, we have developed a novel transformation approach of Schizochytrium using the Agrobacterium tumefaciens (A. tumefaciens) binary vector system.
The h6h gene encoding H6H from Hyoscyamus niger, placed under the regulation of the CaMV 35S promoter, was introduced into the genome of a scopolamine-rich Duboisia hybrid by a binary vector system using the Agrobacterium rhizogenes strain LBA9402.
The pmt gene of Nicotiana tabacum was placed under the regulation of the CaMV 35S promoter and introduced into the genome of a scopolamine-rich Duboisia hybrid by a binary vector system using the disarmed Agrobacterium tumefaciens strain C58C1 carrying the rooting plasmid pRiA4.
As noted in previous research, an ideal binary vector system should include the following features: an easy route for cloning the gene of interest under control of a wide variety of promoter and terminator sequences, the ability to simultaneously express several target genes, and a wide choice of selection markers and reporter genes [16].
The cassette was transferred to pCAMBIA 1302 binary vector system.
Taking advantage of the well established ability of Agrobacteria tumefaciens to transfer a defined segment of DNA (T-DNA) to the plant nucleus [ 4], the molecular genetic modification of plants is most often performed using binary vector systems that separate the T-DNA from the trans-acting virulence proteins that effect the transfer [ 5].
The Nib-hairpin cassette was subcloned into the binary pART7/27 vector system under the control of CaMV 35S promoter [ 54].
A binary transgene vector system was developed based on the 335-amino acid Thm3 transposase and terminal inverted repeats (ITRs) from silver carp nonautonomous element Thm1.
To explore whether the silver carp Thm3 transposase has transposition activity, a binary transposon vector system was developed based on Thm3 transposase and ITRs from the silver carp nonautonomous element Thm1.
In order to study and engineer the biosynthesis of these pharmacologically active metabolites, a binary vector co-transformation system was developed for V. officinalis hairy roots.
