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In addition, numerous commonly used restriction enzyme sites exist on the backbone of the binary vector, limiting the construction strategy with regard to the use of restriction sites.
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The construct was cloned into the binary vector pART27 using the NotI sites [ 33].
Digested PCR products were then ligated into the binary vector to get the final construct.
The binary vector pTM050 contained the hACHE-S gene with a single-base mutation.
Two variants are the super binary vector strategy and the twin T-DNAs strategy.
The P35S::LIF2:GFP and P35S::GFP:LIF2 constructs were then introduced into the pCambia1300 binary vector, generating the binary pCaLIF2 GFP and pCaGFP LIF2 plasmids.
The 22th 24th are the 3-dimensional binary vector for the secondary structure of the residue.
The new construct was inserted into the binary vector at the unique Bsp120I and AscI sites.
Amplified cDNA products were cloned into the binary vector pBI121 by replacing the GUS gene.
The binary vector plasmids were introduced into the Koshihikari cv.
We use a binary vector P to represent the phenotype of these M individuals.
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