Sentence examples similar to binary vector harboring the from inspiring English sources

The construction of a binary plant expression vector harboring the βAS gene was as described elsewhere [ 19].

The pCAMBIA derived binary vectors harboring bp100der cassettes were transferred into Agrobacterium strain EHA105 using the cold shock method [ 89].

This vector harbors the human decorin (DCN) cDNA under the control of cytomegalovirus (CMV) promoter.

Mix 4 ml of exponentially growing suspension-cell culture (approximately 40% of packed cell volume) with 100 µl of the above culture of A. tumefaciens LBA4404 containing the pBIN19 binary vector harboring the chimera cassette.

Agrobacterium tumefaciens strain EHA105 harboring a binary vector pCABIA1301 carrying the UidA reporter gene and hygromycin phosphotransferase gene under a constitutive CaMV35S promoter was used.

Agrobacterium tumefaciens strain EHA105 harboring the binary vector pCAMBIA2300 was used to transform the HRC.

Briefly, ATMT was performed on germinating conidia using the A. tumefaciens strain C58pGV2260 harboring the binary vector pBBH.

Because the BGM vector harbors BAC vector sequences for cloning BAC inserts, this system can be applied to other species.

Later experiments used a hypervirulent derivative of strain LBA4404 [ 37], which harbors the binary vector pSB187; this differs from pYF133 in the promoter chosen to drive HPT (CaMV 35S) and the vector backbone which was derived from pLH6000 [ 38].

All these genomic fragments were cloned into the NT007 entry vector, and then introduced into the binary vector pBGW using the Gateway LR recombination reaction (Invitrogen).

The AtPIP1 4 GFP fusion gene was inserted into the plant binary vector pCAMBIA1301 between the 3′-terminal end of P35S and front of the right border77.