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The topology of a truss is decomposed into a form vector – the placement of cells containing diagonal members – and a binary vector describing the slopes of the diagonals.
The genetic interaction profile for a given gene X was a binary vector describing the existence of genetic interactions between X and all other genes.
The protein domain profile for a given gene X was a binary vector describing the existence of each human protein domain in the IntePro database on gene X.
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The full-length cDNA fused with GFP was cloned into the binary vector described above.
The binary vectors described above were introduced separately into the Agrobacterium tumefaciens strain EHA105 by electroporation [ 50].
The products from the second round of PCR were purified and mixed with any of the binary vectors described in this report to set up the Golden Gate reaction as described above.
Taken together our results suggest that the binary vectors described here should be a valuable addition to the repertoire of viral vectors used in basic and medical research.
The binary vectors described above were introduced into Agrobacterium tumefaciens strain GV3101 pMP90RKK [ 49] by electroporation [ 50].
The binary vectors described above were introduced into Agrobacterium tumefaciens strain AGL1 by electroporation, as previously described in [ 87].
The insert or the purified PCR fragment (T1T2-PCR), together with any of the binary vectors described in this report, were used to set up restriction-ligation reactions, as described elsewhere [ 44], using BsaI and T4 Ligase (New England Biolabs).
When constructing binary vectors carrying one or two gRNAs, only two 23-nt synthetic oligos (annealed to an insert) or a PCR fragment, respectively, are required, along with any of the binary vectors described in this report, to set up Golden Gate reactions.
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