Sentence examples for binary vector containing the from inspiring English sources

pABr1 is a binary vector containing the T-DNA sequence interposed between the left border and the right border.

To overexpress OsPORA in fgl mutant, full-length OsPORA cDNA was subcloned into the pMDC32 Gateway binary vector containing the cauliflower mosaic virus 35S promoter (Curtis and Grossniklaus 2003).

For complementation of the z3 mutation, a full-length cDNA of Z3 was cloned into the pMDC32 Gateway binary vector containing the cauliflower mosaic virus (CaMV) 35S promoter (Curtis and Grossniklaus 2003).

Tobacco leaves were transiently transformed with a binary vector containing the Zera-xylanase coding region, which was optimized for plant expression, under the control of the 35S CaMV promoter.

Amplified cDNAs were sequenced and then cloned in a modified version of the pPZP221 binary vector, containing the 1.3 kb AtMYB60 promoter and the nos terminator (Galbiati, unpublished).

Using unique EcoRV sites the entire promoter cassettes were cloned into pGreen 0229 binary vector containing the bar gene as a selectable marker.

The LIF2 and LHP1 cDNAs were inserted into the binary vectors, containing the split YFP N-terminal fragment (YFPN) and the C-terminal fragment (YFPC) and their expression was analyzed in transiently transformed N. benthamiana leaves [50].

The binary vectors containing the gene constructs described above were transferred to Agrobacterium tumefaciens GV3101 cells.

Binary vectors containing the cDNA of the mammalian glycosyltransferases were co-infiltrated with the viral-based vectors (OD600 of ∼0.15 0.2 for all agrobacteria).

The 35S NTAP AtCTF7∆B construct was generated using Gateway-compatible binary vectors containing the NTAP tag ((NTAPi) [ 30]; a gift from Dr. Qinn Li lab, Miami Univeristy).

Binary vectors containing the full-length cDNA of TRV isolate PpK20 RNA-1 (pTRV1) and RNA-2 (pYL156-GFP) were described previously [ 36, 37].