Sentence examples for binary vector carrying the from inspiring English sources

pCHF3 is a binary vector carrying the cauliflower mosaic virus 35S promoter and a pea ribulose 1,5-bisphosphate carboxylase/oxygenase terminator, and conferring kanamycin resistance for selection in plant [43].

A binary vector carrying the cDNA sequences of both antibody chains was used for agroinfiltration and the yield was 110  μg/g FW (corresponding to approximately 0.5% TSP) in the leaves.

For use in genetic engineering, the Ti plasmid has been divided into a helper plasmid which is devoid of the T-DNA and remains within the Agrobacteria and a binary vector carrying the T-DNA which can be manipulated in E. coli.

Binary vectors carrying the cSUC2 cassettes were transformed into heterozygous AtSUC2 +/- plants because homozygous SALK_038124 (AtSUC2 -/-) plants are unsuitable for floral dip transformation.

Since both binary vectors carried the hygromycin phosphotransferase gene (hpt) as a selectable marker, the transgenic plants were selected on medium supplemented with hygromycin.

For this reason, we added to the binary vector carrying 35S/FECT/GFP another component carrying the nos promoter (Pnos) driving the expression of p19 and terminated by the nos terminator (Tnos).

The transformation binary vector carrying an anti-apoptotic baculovirus p35 gene cassette (CaMV 35S promoter-p35 ORF-Nos terminator) and the op-iap gene cassette (CaMV 35S promoter-op-iap ORF-Nos terminator) was designated p1301 p35-iap (Fig. 1).

By 6 dpi, the fluorescence of the trans configuration (separate binary vectors carrying FECT/GFP and 35S/p19) had increased so as to be somewhat superior to the cis configuration.

Thus, the binary vector set combined with the gRNA module vector set comprise an efficient, inexpensive, time-saving, user-friendly, multifaceted, extensible toolkit for the generation of CRISPR/Cas9 binary vectors carrying one or more gRNAs for targeted mutations of multiple genes.

The binary vector set combined with the gRNA module vector set comprise an efficient, inexpensive, time-saving, user-friendly, multifaceted, extensible toolkit for the generation of CRISPR/Cas9 binary vectors carrying one or more gRNAs for targeted mutations of multiple plant genes.

Binary vectors carrying HPT or CTB were used as positive control of the analysis.