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Electrochemical copolymerization of furan and thiophene was performed at constant electrode potential in a binary solvent system consisting of boron trifluoride-ethyl ether and an additional amount of ethyl ether (mole ratio 1 2).
A binary solvent system consisting of eluent A: 0.1% formic acid in water and B: 0.1% formic acid in acetonitrile (Romil Chemistry, UK) was used.
The mobile phases used were the binary solvent system consisting of fraction A (3% acetonitrile, 2% acetic acid, 95% MilliQ-water) and fraction B (85% acetonitrile, 2% acetic acid, 13% MilliQ-water).
A binary solvent system consisting of solvent A, 2% aqueous acetonitrile with 0.1% formic acid, and solvent B, acetonitrile with 0.1% formic acid was used for the analyses.
The nano-LC system employs a binary solvent system consisting of solvent A, 0.1% formic acid (FA) in 3% ACN in water (v/v), and solvent B, 0.1% FA in 90% ACN in water (v/v).
Separation was achieved on an Eclipse XDB-C8 (4.6 × 150 mm, 5 μm) column (Agilent Technologies) by use of a binary solvent system consisting of (A) 0.05% TFA in water and (B) 0.05% TFA in acetonitrile.
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Binary solvent system consisted of 35% solvent A (0.1% formic acid in H2O, pH: 3.2) and 65% solvent B (acetonitrile) used as mobile phase at flow rate of 0.25 mL with a total run time of 5 min.
After a simple solvent precipitation procedure, separation was achieved using an amide column (2.1 mm by 50 mm, 2.7 μm; Advanced Materials Technology, Wilmington, DE, USA) with a binary gradient solvent system consisting of 2.5 to 10 mM acetonitrile-ammonium acetate at pH 3.5.
The plates were developed in solvent system consisting of Dichloromethane:Ethyl acetate:Methanol Acetic acid (8 4 2 0.1).
Samples were resolved using an initial polar solvent system consisting of 60 35 5 chloroform/methanol/water, followed by a second solvent system containing 70 30 1 hexane/diethyl ether/acetic acid.
Solvent system consisted of 5% MeOH and 95% 50 mM potassium phosphate.
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