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Image segmentation was performed by RGB colour discrimination using threshold ranges such that a binary overlay was created covering only the positively stained areas.
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Imaging was performed, as shown above, using the appropriate fluorescent filters; overlays were created using ImageJ software.
Overlays were created using Adobe Photoshop CS4. Whole-mount immunohistochemistry of zebrafish was carried out as described [ 22].
A binary mask was created from the selected areas, and applied on the initial stack.
A binary indicator was created for each lab result.
Using the age range a binary item was created.
A binary variable was created whereby sometimes, often or never were considered inconsistent condom use.
Binary coding was created (female incubation = 1 and male incubation = 0).
A binary variable was created with current smoker versus former/never smoker.
A binary indicator was created with the not true response equal to 1.
A binary variable (yes/no) was created for each question.
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