The Pitzer model is based on the available experimental data and includes binary interactions between two different kinds of ions of opposite charge (cation M and anion X), and also binary interactions between ions of like charge and ternary interactions involving three ions in common-cation and common-anion ternary systems.
Most of all, only the pairwise binary interactions between two proteins are considered in PPI, and they are not sufficient to capture real biological processes involving a stable form of proteins grouped according to their biological functions.
The resulting network has finally been binarized using a cutoff of five or more common chemicals interactors to set a binary interaction between a pair of genes (Fig. 2b).
This suggests that the interaction between Gro and the target gene is stabilized by more than a binary interaction between Gro and hypoacetylated histone tails.
Gulbis et al (1996) investigated binary interactions between p21 and PCNA and proposed three peptides bound to trimeric PCNA within the crystal structure.
Binary interactions between DVAP and Sac1 were performed using the MATCHMAKER 3 GAL4 Yeast Two Hybrid System (Clontech).
These five binary mixtures are the representative of binary interactions between alkane, one-ring aromatic and two-ring aromatic hydrocarbons.
Despite being unable to observe binary interactions between RGS14/B-Raf, RGS14/MEK1, or RGS14/ERK1, RGS14 appears to assemble a stable, multiprotein complex containing H-Ras, B-Raf, MEK1, and ERK1 when all five proteins are expressed concomitantly (Figure 5).
