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Accordingly, the planar occupation area of the bright parts expressed as 1 in the corresponding binary image, was summed.
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The three binary images are summed to create a new composited image (Fig. 2e).
Briefly, the ten consecutive z-stack images were summed, the summed image was duplicated, converted to 8-bit grayscale, and then thresholded to produce a binary image (Additional file 1: Figure S6, and S7).
The ATase positive binary image was added to the propidium iodide positive binary image and the total nuclear area recorded.
A binary image was obtained from the previous step, and red patches were labeled from the binary image.
A binary image was obtained by threshold function.
Transformation to binary image is used in [19].
This binary image is used to extract contour roughness features.
The final binary image is displayed as (e).
Then the binary image is detected by Hough line.
The binary threshold value of the mean + 0.5σ was applied, and the resulting binary image is shown in Fig. 11c.
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