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The largest connected component of the binary image was identified as the cell cluster.
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4) Using a thresholding, or image segmentation process, the intensity of pixels within the square is measured, and another rough nuclear region (binary image) is identified.
The centroids of the bright spots in the binary images were identified by using the image processing toolbox in MATLAB (Mathworks, Inc ., thereby coarsely locating the single droplets in the differential grayscale images.
Not all images are identified.
A binary image was obtained by threshold function.
The ATase positive binary image was added to the propidium iodide positive binary image and the total nuclear area recorded.
The binary image is cleaned and thinned to a fiber skeleton representation of the image.
The binary threshold value of the mean + 0.5σ was applied, and the resulting binary image is shown in Fig. 11c.
Binary toxin was identified in 12 (38.7%).
Conversion to binary images was done using ImageJ.
As a result of this process, the binary image object representing the canal impression was identified and isolated from the background.
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