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The pixels that lie in that region are assigned to value 1 and all the remaining pixels are made to 0. Now, gray image is converted into binary image in which road regions appeared in white and all other pixels appeared in black.
The segmentation procedure (phase A, routine: A_Imagefilters.m) consists of the application of four consecutive filters and provides a binary image in which single saturated pixels correspond to the discerned target positions.
Interstitial fibrosis was quantified using a computer-based morphometric analysis software (Analysis, Olympus) that allowed the formation of a binary image in which the stained area could be automatically calculated as percentage of the image area.
Zero-crossing detection in the resulting image provides a binary image in which dark regions represent the bone marrow and light regions represent trabeculae [ 9].
The output of the local thresholding is a binary image in which the potential follicle regions are marked as positive (i.e. 1).
Class merging transforms the five level output of the Otsu's algorithm, pre-filtered with a median filter that ensures discontinuities in the meniscus profile, to a binary image, in which the last four classes are combined.
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Edge detection and filter are carried out to obtain binary images in which the edges of the buckle are obvious, and false noise is eliminated.
Binary images in which cell was in black against a white background were obtained using the threshold.
The RGB images were converted into binary images, in which the vascular pixels and perivascular pixels were discriminable based on their gray-scale intensities.
Lesion membership matrix is now converted to a binary image which shows normal voxels in black and lesion voxels in white.
The extracted compound structure phase was then represented in red-based pseudo-color to produce a binary image, termed an extracted PD image (ePD image), in which red patches mostly represent granular spots in cytoplasm.
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