The general users select from binary gradient programs after running an analytical TLC with the raw product.
dFdCMP, dFdCDP and dFdCTP were confirmed by HPLC (Shimadzu Scientific Instruments, Braintree, MA, USA) using a binary gradient of water and 0.3 M potassium phosphate buffer in an anion exchange column (Partisil-SAX, Whatman, Inc., Clifton, NJ, USA).
Moreover, with binary gradient elution using SDS/DOC cosurfactant solution, s-SWCNTs can be further separated according to chirality.
The binary gradient elution (A B proportion, v/v), at a flow rate of 0.3 ml.min−1, was composed by 96:4 from 0 to 5 min; switching to 50 50 from 5 to 7 min; maintained by 11 min; switching back to the initial condition from 11 to 13 min; and maintaining on this proportion till 16 min.
A binary gradient consisting of (A) 20 mM ammonium acetate and 0.1% acetic acid in water, and (B) 20 mM ammonium acetate and 0.1% acetic acid in 85:15 (v/v) methanol/isopropanol was used to separate the GSLs at a flow rate of 0.3 ul/min.
Binary gradient elution is used for the rapid separation of semiconducting (s-) single-walled carbon nanotubes (SWCNTs) with single or few chiralities using single-column sephacryl gel chromatography.
This paper proposes a binary gradient method for analyzing biodiesel mixtures using non-aqueous reverse phase HPLC with a UV detector capable of overcoming the drawbacks of the gas chromatographic technique normally used.
The baseline separation of these amino acid derivatives was obtained on a C8 column with a binary gradient elution using the mobile phase of 20 mmol/L mixed acid buffer solution (pH 6.40) and methanol with 2.5% tetrahydrofuran (THF).
Figure 1 Solvent B concentration-time profile of a 20-min LC binary gradient program.
A binary gradient was used for H2O (Solvent A) and methanol (Solvent B).
The binary gradient elution system consisted of 0.001% phosphoric acid in water (A) and 0.001% phosphoric acid in acetonitrile (B).
