Sentence examples for binary destination from inspiring English sources
The coding regions were recombined into binary destination expression vectors pH7RWG2 (Cauliflower mosaic virus (CaMV) 35S promoter-driven) for C-terminal RFP (red fluorescent protein) fusions39,40 or pSITE-2CA and pSITE-2NA (2 × 35S) for N-terminal and C-terminal GFP (green fluorescent protein) fusions, respectively41.
For the transient assay, the ORF region, of AtBS14b was recombined into the pEarlyGW 104 binary destination vector.
The 1.5 Kb fragment (111…1633 nt). of MIPS gene from the entry clone (pENTR-MIPS) was then introduced into the binary destination vector, pIPKb006 (Himmelbach et al. 2007) using LR clonase (Invitrogen, USA) based recombination reaction.
The open reading frame (ORF) regions of AtBS14b (andG14455) AtBS14aS14a (AT3G58170) were recombined into the pABind118 GW binary destination vector via a Gateway LR reaction, and Agrobacterium-mediated transformation into Arabidopsis thaliana WS2 ecotype was used to generate AtBS14b overexpressing transgenic plants (Bleckmann et al. [2010]).
Subsequently an LR recombination reaction was carried out to introduce the fragment into the pBI101-R1R2 binary destination vector (F. Divol, J.-C.
Validated entry clones were recombined with binary destination vectors pB7FWG2,0, pB7RWG2,0 or pB7YWG2,0 clone [93] providing expression from Agrobacterium T-DNA, using the cauliflower mosaic virus 35S promoter upstream of coding fusions to green fluorescent protein (GFP), red fluorescent protein (RFP) or yellow fluorescent protein (YFP), respectively.
To evaluate the application value of thanatin(S) in plant disease control, the synthesized coding sequence of Cht1 signal peptide (Cht1SP -thanatin(S) was ligated to plant gateway destination binary veCht1SP -thanatinth FLAG tag).
The fragment was transferred to a binary Gateway® destination plasmid, pAGSM552 (GenBank accession KP259613) using LR Clonase II recombination (Life Technologies).
The resulting plasmids were then recombined into the destination binary vector pGWB13 or pGWB5 (Nakagawa et al., 2007) for Arabidopsis transformation or transient expression in N. benthamiana.
This construct was thereafter used for LR-mediated recombination of PpHsp16.4 cDNA sequence into the pK7FWG2 destination binary vector [ 75], containing the GFP coding sequence under the regulation of the 35S promoter.
The GCL and TSR coding sequences were synthesized with flanking att sites and cloned in the destination binary vector pEarleyGate100 (pEG100) via gateway-assisted recombination [ 76], resulting in vector TG1 with a bar selectable marker gene [ 77, 78] (Fig. 2a).
