Sentence examples for binary construct were used from inspiring English sources

CrMPK3/CrMPK3 K69R in binary construct were used for transformation of competent cells of Agrobacterium tumefaciens strain GV3101.

The same binary construct was used for the apple transformation.

The binary construct 35S::MdCIbHLH1 was introduced into Agrobacterium tumefaciens GV3101, and Arabidopsis plants were transformed using the flower dipping method described by [ 36]. A. tumefaciens strain LBA4404 containing either the 35S::MdCIbHLH1:GFP or 35S::GFP binary construct was used to transform apple calluses.

This construct was used for pronuclear injections.

Binary constructs were then used to transform Agrobacterium tumefaciens strain GV3101 carrying the helper plasmid pJIC Sa_Rep (pSoup).

Agrobacteria carrying the magnICON® constructs were infiltrated using OD600 0.05–0.2 and those carrying the other binary constructs were infiltrated using OD600 0.05 (1.0 OD600 corresponds to 5 × 10 cells/mL).

Binary constructs were introduced into Agrobacteria and used to transform the corresponding plant cultivars [46], [47].

Two further binary definitions were used.

In binary outcomes χ tests were used.

The 35S NTAP AtCTF7∆B construct was generated using Gateway-compatible binary vectors containing the NTAP tag ((NTAPi) [ 30]; a gift from Dr. Qinn Li lab, Miami Univeristy).

Crystal structures of the binary complex (PDB ID: 2HMY [ 7]) were used to construct the protein model, in which all water molecules were removed.