Sentence examples for binary construct was used from inspiring English sources
The same binary construct was used for the apple transformation.
The binary construct 35S::MdCIbHLH1 was introduced into Agrobacterium tumefaciens GV3101, and Arabidopsis plants were transformed using the flower dipping method described by [ 36]. A. tumefaciens strain LBA4404 containing either the 35S::MdCIbHLH1:GFP or 35S::GFP binary construct was used to transform apple calluses.
CrMPK3/CrMPK3 K69R in binary construct were used for transformation of competent cells of Agrobacterium tumefaciens strain GV3101.
Binary constructs were then used to transform Agrobacterium tumefaciens strain GV3101 carrying the helper plasmid pJIC Sa_Rep (pSoup).
Agrobacteria carrying the magnICON® constructs were infiltrated using OD600 0.05–0.2 and those carrying the other binary constructs were infiltrated using OD600 0.05 (1.0 OD600 corresponds to 5 × 10 cells/mL).
The 35S NTAP AtCTF7∆B construct was generated using Gateway-compatible binary vectors containing the NTAP tag ((NTAPi) [ 30]; a gift from Dr. Qinn Li lab, Miami Univeristy).
The CaMV 35S promoter in binary vector pBI121 was used to construct hG-CSF chimeras and the phaseolin signal peptide was introduced to direct hG-CSF into the plant cell secretory pathway.
The RNAi-HMGR construct was designed using the pHANNIBAL vector and subsequently cloned into a binary vector pART27.
The binary construct pCCreloxPBt [ 34] was used for MD44 transformation.
The binary vector, pART27 was used for both the overexpression and silencing constructs [ 34].
