Sentence examples for binary clone from inspiring English sources
noun
A composite organism created from the combination of DNA from two separate genetic sources, rather than one. Essentially, a binary clone is the naturally produced offspring with germ cells, or else artificially produced offspring of two individuals that has been created without using germ cells.
Rps1-k-3 was identified from a binary clone, p43-10 cloned in the pTF101.1 vector [ 1].
Comparison of the insert sequence of p43-10 with GS_43D16 sequence revealed that the insert of the binary clone carries sequences identical to a BamHI fragment that contains an internal BamHI site.
A contig of binary clones from IR40931-26, a submergence tolerant FR13A derivative, was also assembled across this region.
The positional cloning of SUB1 was accomplished by identifying a contiguous set of BAC and binary clones spanning the region, using the intolerant indica Teqing and the tolerant FR13A derivative IR40931-26, respectively.
Binary clones in Agrobacterium tumefaciens GV3101 were used for plant transformation [95].
The clone was isolated from a library of binary clones prepared from GS_43D16 DNA partially digested with BamHI.
Bacteria have in the past been regarded as very simple organisms, dividing by binary fission into clones of identical daughter cells which can perform an unlimited number of cell divisions and thus were considered immortal in the absence of external killing events.
This system included a series of vectors for gene cloning, promoter cloning, and binary vector construction to meet various needs for plant functional genomic study.
All basic vectors including gene entry clones, promoter entry clones, and binary destination vectors, were directly synthetized according to the sequences on Additional file 1.
BDV: Binary destination vector; EC: Entry clones; GEC: Gene entry clone; NES Nuclearr export signal; NLS: Nuclear localization signal; PEC: Promoter entry clone; REL: Restriction enzyme/ligase; SSR: Site-specific recombination; SUC2: SUCROSE H+ SYMPORTER2.
The engineered constructs consisting of PCR-amplified DNAs were cloned into binary vector pJL89 immediately downstream of a double cauliflower mosaic virus (CaMV) 35S promoter, and upstream of the hepatitis delta virus (HDV) ribozyme and nopaline synthase terminator (NOS).
