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For analysis, the hemolymph of Daphnia was considered as a binary buffer system consisting of the carbonate system and a monoprotic non-bicarbonate buffer (HA ↔ H+ + A-).
Reverse phase chromatography was performed with a binary buffer system consisting of buffer A (0.1% formic acid, 2% acetonitrile in water) and buffer B (0.1% formic acid in acetonitrile).
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For HILIC, the buffer system consisted of (A) 25 mM ammonium acetate in water and (B) acetonitrile.
Peptides were separated on nanoLC 1000 (Proxeon, now Thermo Fisher) using a binary buffer system of A (0.1 % (v/v) formic acid in H2O) and B (0.1 % (v/v) formic acid in 80%Acetonitrile 699.
An EASY-nLC system was connected to the mass spectrometer with a nanospray ion source, and peptides were separated with a binary buffer system of 0.5% acetic acid (buffer A) and 80% MeCN plus 0.5% acetic acid (buffer B), at a flow rate of 250 nL/min.
A binary star system consisting of a black hole and a normal star, located about 11,000 light years from Earth.
A binary solvent system consisting of eluent A: 0.1% formic acid in water and B: 0.1% formic acid in acetonitrile (Romil Chemistry, UK) was used.
Didymos is actually a binary asteroid system consisting of two separate space rocks bound together by gravity.
Sirius is a binary star system consisting of two white stars orbiting each other with a separation of about 20 AU (roughly the distance between the Sun and Uranus) and a period of 50.1 years.
It is a binary star system, consisting of a yellow-white main sequence star of spectral type F5 IV-V, named Procyon A, and a faint white dwarf companion of spectral type DA, named Procyon B.
A binary solvent system consisting of solvent A, 2% aqueous acetonitrile with 0.1% formic acid, and solvent B, acetonitrile with 0.1% formic acid was used for the analyses.
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