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For spectral imaging, the camera was used in 2 × 2 binning mode in order to capture monochromatic images.
In this experiment, the CCD was operated in the 8×8 binning mode, and the actual pixel number was 256×256.
In the actual measurement, a CCD camera was used in the binning mode (4×4 binning with 23.5 µm pixels).
The spatial resolution was either 0.5 mm or 1 mm in binning mode.
The detector was operated in 2-by-2 binning mode, resulting in an effective pixel size of 58 μm.
SPLASSH communicates directly with Firewire cameras over the Firewire bus to manipulate camera properties (e.g. frame rate, exposure time, spatial binning mode, etc).
Table 1 summarizes the binning mode including the pixel size and the acquisition time for each image.> Spectra of BVs were retrieved from integration false colour maps based on the ratio of protein (1700 1500 cm−1) to the CH stretching region (3000 2800 cm−1) as this helped to reveal BV structure (since this is not always evident in the unstained tissue section).
(Some of these have that sweet little sensor with its special pixel binning modes (pro-low light mode).
The illumination time was 250 ms and binning modes 2·2.
For this sample, we tried different binning modes (2 × 2, 4 × 4 and 8 × 8).
First, we assessed the FPA performances using different binning modes by quickly screening mouse tumour tissue sections as a test to determine which binning option is the best.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com