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2. Liver function tests: Serum levels of aspartate transaminase (AST), alanine transaminase (ALT), albumin, total and direct bilirubin were done using Integra-400 (Roche-Germany).
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Serum biochemistries were done using the Hitachi 737 automated multichannel chemistry analyser (Boehringer Mannheim Diagnostics, Indianapolis, IN). 26 Liver tests determined levels of aspartate aminotransferase, alanine aminotransferase, γ-glutamyltransferase, alkaline phosphatase, and total bilirubin.
Posttreatment studies were done in all newborns until total serum bilirubin levels had decreased to less than 171 μmol/L.
Associations involving liver enzymes and bilirubin were analysed using Spearman's rank correlation coefficient.
Magnesium, calcium, phosphate, albumin, creatinine and total bilirubin were measured using freeze-thawed stored serum samples.
In addition lipid profile was done in all the patients with normal serum bilirubin levels.
Serum total bilirubin was measured using the Bilirubin Total DPD Gen.2 kit (Roche, Mannheim, Germany).
Because no patient developed veno-occlusive disease, increased serum bilirubin was used to characterize hepatotoxicity.
Total bilirubin was used in the present study.
Serum unconjugated bilirubin concentrations were analyzed using high-performance liquid chromatography (HPLC) (see Bilirubin and heme analysis).
Levels of serum albumin (ALB), ALT, aspartate transaminase (AST), and total bilirubin (TB) were determined using a Hitachi 7600 analyzer (Hitachi Ltd., Tokyo, Japan).
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