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Firstly, having the FRAP system on the vertical axis and the bilayer set-up on the horizontal axis.
At the initial stages of discussing the concept of combining the FRAP and bilayer set-up, two configurations were discussed.
Introduction of the bilayer set-up involved the designing and construction of a bilayer chamber which was compatible with the FRAP set-up.
After selective etching of the sacrificial layer, the bilayer was set free and the strain gradient causes rolling of the bilayer nanomembrane into microtube [12].
The trans side of the bilayer was set as ground.
At a given detergent concentration, two thresholds referred to as the solubilization (SOL) and saturation (SAT) boundaries indicate the lipid concentrations at which bilayer formation sets in and is completed, respectively.
The ratio of core DOTAP to the bilayer HPC was set to 0.7.
LPS is a glycolipid consisting of a 'lipid anchorhor within the bilayer, and a set of covalently attached distal 'core' saccharides (Raetz and Whitfield, 2002).
The strategy is based on the integration of the pressure sensing β- BEDT-TTF 2I3/polycarbonate bilayer film and a set of gold electrodes into one compact multi-layer design.
These results are a consequence of the increasing thickness, which is around 600 μm in the case of the film formed by 100 bilayers of the second set; the roughness could also contribute to the scattering of light, increasing the optical transmission losses.
The results indicate that under a given set of conditions, a bilayer cover (with a capillary break above the reactive tailings) would not significantly improve cover performance (compared to a monolayer cover) due to water losses by evaporation.
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