This is because, in the presence of 2.5 mM indole or 50 nM CCCP the leakage ionic conductance of the lipid bilayer, representing the ions that pass the lipid bilayer not being carried by the ionophore, is not negligible compared to the ionophore induced conductance [19].
For most chemicals, the route across lipid bilayers represents the main diffusional pathway.
Regardless of how the planar membrane interacts with the solid support, display of these lipid bilayers represents a powerful way of developing technologies ranging from biosensors [ 4, 7] to platforms for the study of ion channel electrophysiology [ 8].
Image processing of extracted sub-tomograms depicted the architecture of the bipartite complex sandwiched between two lipid bilayers, representing an environment close to that of the native whole pump (i.e. anchored between outer and inner membranes of P. aeruginosa).
SPR studies using a Biacore L1 biosensor chip (36, 37, 49) were used to gain insight into interactions of ramoplanin with phospholipid bilayers representing mammalian and bacterial membranes.
We believe that monitoring protein-induced charge flux across a bilayer membrane represents a universal method for quantitatively monitoring ion-channel activity.
The free energy of transferring an ion from bulk water to the lipid bilayer computed from the atomistic simulations indicates that large errors are introduced when the bilayer is represented as a single slab of low dielectric embedded in the higher-dielectric solvent.
The lipid bilayer was represented by two superimposed triangular lattices with skew-periodic boundary conditions (60).
In these simulations the lipid bilayer is represented by two superimposed triangular matrices, each site representing a lipid.
